Journal: Materials Today Bio

Article Title: Biomimetic 3D-Bioprinted organoids of thymic epithelial tumors for translational drug screening and biomarker identification

doi: 10.1016/j.mtbio.2026.102878

Figure Lengend Snippet: (A-B) H&E staining of primary TC tissue and corresponding 3DP organoids.Scale bars: 50 μm (C) Immunofluorescence images of TC organoids stained for DAPI, CD56, CD117, and pan-CK. Scale bars: 50 μm (D-E) H&E staining of primary THYM tissue and corresponding 3DP organoids. Scale bars: 50 μm (F) Immunofluorescence images of THYM organoids stained for DAPI, P63, pan-CK, and TdT.Scale bars: 100 μm. (G) CNV profiles of TC-Tumor. (H)TC-3DP organoids (I) TC-Matrigel organoids. (J) CNV profiles of THYM-Tumor. (K) THYM-3DP organoids. (L)THYM-Matrigel organoids.

Article Snippet: The following primary antibodies were used: Pan-CK (# MA513203 , invitrogen) at a dilution of 1:100, CD117 (AF1356-SP, R&D system) at a dilution of 1:100, CD56/NACM1 (#ab313779, abcam) at a dilution of 1:100, p63 (#ab124762, abcam) at a dilution of 1:100, TdT (#TB4932S, abmart) at a dilution of 1:100, and Anti-gamma H2A.X (phospho S139) antibody (#ab2893, abcam) at a dilution of 1:100.

Techniques: Staining, Immunofluorescence

Journal: BMC Cancer

Article Title: Subtyping atypical eosinophilic renal cell tumours through integrated morphological, immunohistochemical, and mutational characters

doi: 10.1186/s12885-026-15870-1

Figure Lengend Snippet: Histopathological and immunohistochemical features of atypical eosinophilic renal cell tumours. A1–A6: Renal oncocytoma. A1: The tumour is well-circumscribed from the surrounding tissue and unencapsulated. A2: The tumour exhibits solid and nested/sheet-like growth with intervening edematous stroma. A3: CK7 demonstrates focal strong cytoplasmic positive. A4: CD117 shows diffuse weak cytoplasmic positive. A5: CK20 is negative. A6: Colloidal iron staining is negative. B1–B6: Eosinophilic chromophobe renal cell carcinoma. B1: The tumour is relatively well-circumscribed, without involvement of the renal sinus fat. B2: Solidly packed tumour cells include distinct populations with botryoid morphology and eosinophilic cytoplasm. B3: CK7 shows diffuse strong cytoplasmic positive. B4: CD117 shows diffuse strong cytoplasmic positive. B5: CK20 is negative. B6: Colloidal iron staining is positive. C1–C6: Low-grade oncocytic tumour. C1: The tumour is well-circumscribed and unencapsulated. C2: Solid and acinar growth patterns are observed. C3: CK7 shows diffuse strong cytoplasmic positive. C4: CD117 is negative. C5: CK20 is negative. C6: Colloidal iron staining is negative. D1–D6: Eosinophilic vacuolated tumour. D1: The tumour is relatively well-circumscribed from the adjacent normal tissue, with scattered proliferative blood vessels within the tumour. D2: The tumour is arranged in solid nests and cords. D3: CK7 shows focal weak cytoplasmic positive. D4: CD117 shows diffuse moderate cytoplasmic positive. D5: CK20 is negative. D6: Colloidal iron staining is negative. E1–E6: Eosinophilic solid and cystic renal cell carcinoma. E1: The tumour is well-circumscribed, unencapsulated, and exhibits a cystic and solid structure. E2: The tumour shows cystic and solid growth patterns. E3: CK7 is negative. E4: CD117 is negative. E5: CK20 shows focal strong cytoplasmic positive. E6: Colloidal iron staining is negative. F1–F6: Normal renal tissue (Control). F1: Normal renal tissue, showing renal tubules, glomeruli, and thick-walled vessels. F2: Normal renal parenchyma. F3: CK7 shows diffuse strong cytoplasmic positive. F4: CD117 shows diffuse strong cytoplasmic positive. D5: CK20 is negative. F6: Colloidal iron staining is negative. Hematoxylin and eosin (H&E) staining x20 (A1-F1) and x40 (A2-F2). EnVision for CK7, CD117 and CK20 × 200. Colloidal iron staining x200

Article Snippet: The antibodies used in the study are CK7 (EP16, Origene, Wuxi, China), CK20 (EP23, Origene, Wuxi, China), CD117 (EP10, Origene, Wuxi, China), mTOR (ab109268, Abcam, Shanghai, China), Vimentin (UMAB159, Origene, Wuxi, China), CAIX(H-11, Origene, Wuxi, China), SDHB (OTI1H6, Origene, Wuxi, China), FH (OTI1F10, Origene, Wuxi, China), Cathepsin K (ab37259, Abcam, Shanghai, China) and Pax8 (OTI6H8, Origene, Wuxi, China).

Techniques: Immunohistochemical staining, Staining, Control